5-HYDROXYINDOLE ACETIC ACID, URINE RANDOM

Specimen Required
Patient Preparation: Patients should not eat avocados, bananas, butternuts, cantaloupe, dates, eggplant, grapefruit, hickory nuts, honeydew, kiwi fruit, melons, nuts, pecans, pineapple, plums, tomatoes, or walnuts for a 48 hour period prior to start and duration of collection. Could result in falsely elevated 5-HIAA excretion. Medications affecting metabolism of serotonin must be avoided at least 72 hours before and during collection. Patients should also avoid coffee, tea and tobacco.
Collect random urine. Add 1-2 drops 6N HCl to a 10.0 mL urine aliquot (5.0 mL minimum) to ensure pH between 1-3. Mix well and send refrigerated in a screw-capped plastic urine container.

Alternate Specimen
Alternate method of collection for random urine specimen without preservative: Send 15.0 mL urine (10.0 mL minimum) refrigerated in a screw-capped plastic urine container.

Methodology
High Performance Liquid Chromatography

Stability
With preservative: Room Temperature: 10 days; Refrigerated: 14 days; Frozen: 1 year
Without preservative: Room Temperature: Unacceptable; Refrigerated: 7 days; Frozen: 14 days

Reference Range
<10 mg/G creatinine

Performed
Monday, Wednesday, Friday

Turnaround Time4-6 days
Test CodeU5HIR
CPT-4 Code (s)
83497
LOINC Codes
Creatinine   2161-8  
5HIA   11145-0  

Warde
Medical
Laboratory