ADENOVIRUS PCR

Specimen Required
Specimen source required.
Swabs (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen-20°C).
Bronchoalveolar lavage/wash in sterile IATA-approved screw-capped plastic container 1.0 mL (0.5 mL minimum).
CSF and body fluid undiluted in sterile IATA-approved screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirated in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in IATA-approved screw-capped plastic container. 1.0 mL (0.5 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum)
Bone marrow in EDTA (lavender top) 1.0 mL (0.5 mL minimum)
Urine undiluted in an IATA-approved screw-capped plastic container. 2.0 mL (1.0 mL minimum)
Stool in sterile IATA-approved screw-capped plastic container 2.0 mL (1.0 mL minimum) for liquid stool or marble -size solid stool

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Serum
Whole blood

Methodology
Polymerase chain reaction (PCR). This method is genus specific and cannot distinguish adenoviral subtypes. The analytical sensitivity of this assay is 200 DNA copies/mL.

Stability
Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeAVPCR
CPT-4 Code (s)
87798
LOINC Codes
Specimen source:   31208-2  
Adenovirus PCR:   39528-5  

Warde
Medical
Laboratory