AFB (ACID FAST BACILLI) CULTURE AND SMEAR

Test Documentation
Specimen Required
Collect sputum from a deep cough. Send 5.0 mL sputum (2.0 mL minimum) refrigerated in a sterile screw-capped plastic container. Collect 3 consecutive specimens 8-24 hours apart. At least one of the three specimens should be an early morning specimen. Source of specimen is required.

Alternate Specimen
Urine (clean catch midstream or catheter collection): 40.0 mL (5.0 mL minimum)
Body fluid, CSF or aspirates: 3.0 mL (1.0 minimum)
Stool from immunocompromised or HIV patient
Tissue (1-2 cm x 1-2 cm piece recommended) - place tissue and needle biopsies in Middlebrook broth.
Nasopharyngeal swab, gastric aspirate

Rejection Criteria
Pooled or 24 hour urine, pooled sputum specimens

Methodology
Fluorescent stain and culture

Stability
Tissue: Room Temperature: 72 hours; Refrigerated: 7 days; Frozen: Unacceptable
All other samples: Room Temperature: 24 hours; Refrigerated: 7 days; Frozen: Unacceptable

Reference Range
Negative

Performed
Sunday-Saturday

Turnaround TimeCulture: Up to 8 weeksStain: 48-72 hours
Test CodeAFBSM, SPAFB
CPT-4 Code (s)
87116, 87206 (smear) and 87015 (concentration) billed separately.
CPT codes for identification and susceptibility vary by method.
If culture is positive for Mycobacterium species, it will reflex to AFB Identification by DNA (GenProbe) or AFB Identification by HPLC at an additional charge. If M. tuberculosis complex is isolated susceptibility testing will be performed at an additional charge.
For other Mycobacterium species, susceptibility testing will be performed upon request.
LOINC Codes
Specimen source: 31208-2
Acid fast bacilli stain 11545-1

Warde
Medical
Laboratory