BK VIRUS DNA PCR, QUANTITATIVE, PLASMA

Specimen Required
Draw blood in a lavender EDTA tube. Centrifuge and separate plasma from cells within 8 hours of collection. Send 2.0 mL plasma (1.0 mL minimum) frozen in a screw-capped plastic vial.
Dedicated specimens are required. Specimens used in other assays will not be tested.

Rejection Criteria
The following specimens will not be tested:
Serum specimens, heparinized specimens, specimens in any gel-based separation media, shared specimens, specimens submitted to repeated freeze-thaw cycles, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above.

Methodology
This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection and quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL (2.10 to 6.70 log(10) copies/mL). The qualitative limit of detection is 100 copies/mL (2.00 log(10) copies/mL).
Specimens reported as "DETECTED" but <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A "NOT DET" (NOT detected result does not rule out infection.

Stability
Room Temperature: 8 hours; Refrigerated: 3 days; Frozen (-20°C): 30 days; Frozen (-70°C): 6 months

Reference Range
BKV QUAL: Not detected (NOT DET)
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL

Performed
Monday-Friday

Turnaround Time1-3 days
Test CodePBKQN
CPT-4 Code (s)
87799
LOINC Codes
BK Virus DNA, Qualitative, Plasma   32362-6  
BK Virus DNA, Quantitative, Plasma   32284-2  
Log BK Virus DNA, Plasma   44805-0  

Warde
Medical
Laboratory