BK VIRUS PCR, QUANTITATIVE, URINE

Specimen Required
Collect random urine: Send 2.0 mL urine (1.0 mL minimum) undiluted, and frozen in a sterile IATA-approved screw-capped plastic container.
Dedicated specimens are required. Specimens used in other assays will not be tested.

Rejection Criteria
The following specimens will not be tested:
Specimens in any gel-based separation media, shared speicmens, specimens submitted to repeated freeze-thaw cycles, urine in Grey-top (boric acid) urine Vacutainers, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above.

Methodology
This test utilizes a multi-target polymerase chain reaction to amplify sequences from the small T antigen and the VP1 regions of the BK virus genome. Real-time detection & quantification are used to determine the viral copy number. The analytical measurement range is 250 to 50 billion copies/mL. Specimens with greater than 5 million copies/mL are diluted to establish the endpoint.

Stability
Room Temperature: 8 hours; Refrigerated: 3 days; Frozen (-20°C): 30 days; Frozen (-70°C): 6 months

Reference Range
BKV QUAL: NOT DET
BKV QUANT: <250 copies/mL
Log BKV: <2.40 log(10) copies/mL

Performed
Monday-Friday

Turnaround Time1-3 days
Test CodeUBKQN
CPT-4 Code (s)
87799
LOINC Codes
48309-9

Warde
Medical
Laboratory