CATECHOLAMINES, FRACTIONATED, PLASMA

Test Documentation
Components
Includes Epinephrine, Norepinephrine, Dopamine and Total Catecholamines

Specimen Required
Patient Preparation: Patients should be relaxed in either a supine or upright position before blood is drawn. States of anxiety and stress can cause fluctuations in the catecholamine levels. Draw specimen in a pre-chilled green-top vacutainer. Patient should avoid alcohol, coffee, tea, tobacco and strenuous exercise prior to collection. Overnight fasting is required. Draw blood in a pre-chilled green sodium heparin tube. Centrifuge in a refrigerated centrifuge within 30 minutes of collection remove plasma from cells and send 4.0 mL plasma (2.5 mL minimum) frozen in a screw-capped plastic vial.
Collection Instructions: Insert an intravenenous catheter and reassure the patient as he/she rests in a supine position for 30 minutes in a quiet room. At the end of 30 minutes, draw 10 mL of blood into each of 2 pre-chilled vials containing heparin (green top). Separate plasma in a refrigerated centrifuge at 4°C, or separate tubes while still cold in a regular centrifuge within 30 minutes after phlebotomy. Send 3.0 mL (1.5 mL minimum) frozen in 2 screw-capped plastic vials. (store at -70°C if possible) CRITICAL FROZEN.

Alternate Specimen
Plasma: Lithium heparin

Methodology
High Performance Liquid Chromatography with Electrochemical Detection

Stability
Room Temperature: 6 hours; Refrigerated: 6 hours; Frozen: 30 days

Reference Range
Epinephrine:   3-15 years:   Supine: < or = 464 pg/mL  
      Upright: No reference range  
Epinephrine:   Adult:   Supine:   <50 pg/mL, Upright:   <95 pg/mL  
Norepinephrine:   3-15 years:   Supine:   < or = 1251 pg/mL  
      Upright:   No reference range available  
Norepinephrine:   Adult:   Supine:   112-658 pg/mL  
      Upright:   217-1109 pg/mL  
Dopamine:   3-15 years:   Supine:   <60 pg/mL  
      Upright:   No reference range available  
Dopamine:   Adult:   Supine:   <10 pg/mL  
      Upright:   ,20 pg/mL  
Total Catecholamines:   Supine:   123-671 pg/mL  
   Upright:   242-1125 pg/mL  

Performed
Monday-Friday

Turnaround Time4-7 days
Test CodeCATFP
CPT-4 Code (s)
82384
LOINC Codes
Total Catecholamines   2056-0  
Dopamine   2216-0  
Epinephrine   2230-1  
Norepinephrine   2666-6  

Warde
Medical
Laboratory