CATECHOLAMINES, FRACTIONATED, URINE - 24 HOUR

Test Documentation
Specimen Required
Collect 24 hour urine (see collection instructions below). Send a 25 mL acidified aliquot (10.0 mL minimum) refrigerated in a screw-capped plastic urine cup. Record total volume on test requisition and specimen label.
24 hour urine collection: The bladder must be emptied at the time the collection is to begin, and this urine is discarded. Thereafter all urine must be collected until the end of the scheduled time.
Preferred method of collection for 24 hour collection: Collect urine with 25 mL of 6N HCl at start of collection to maintain pH between 1 and 3. Refrigerate urine during collection (critical). Mix the collection well before removing aliquot and refrigerate aliquot.
NOTE: Medications that may interfere with catecholamines and metabolites include amphetamines and ampehtamine-like compounds, appetite suppressants, bromocriptine, buspirone, caffeine, carbidopa-levopa (Sinemet), clonidine, dexamethasone, diuretics, ethanol, isoproterenol, methyldopa (Aldomet), MAO inhibitors, nicotine, nose drops, propafenone (Rythmol), reserpine, theophylline, tricyclic antidepressants, and vasodilators.

Alternate Specimen
Alternate method of collection for 24 hour collection: Refrigerate urine during collection (critical) and add 25 mL 6N HCl to the entire collection within 4 hours of completing collection. Mix the collection well before checking the pH. Ensure pH is between 1 and 3. Record total volume on test request and on container label. Send 25 mL aliquot (10 mL minimum). Refrigerate.
If other tests are ordered requiring pH other than 1-3 you may acidify a 25.0 mL aliquot of the 24 hour collection.

Methodology
High Performance Liquid Chromatography

Stability
With Preservatives: Room Temperature: 72 hours; Refrigerated: 7 days; Frozen: 1 month
Without Preservatives: Not stable at any temperature

Reference Range
Epinephrine:
   Age   Epinephrine mcg/day  
   <1 year   0-3  
   1 year   0-4  
   2-3 years   0-6  
   4-9 years   0-10  
   10-15 years   1-20  
   >=16 years   0-20  
Norepinephrine:
   Age   Range  
   <1 year   0-10 mcg/day  
   1 year   1-17 mcg/day  
   2-3 years   4-29 mcg/day  
   4-9 years   8-45 mcg/day  
   10-15 years   13-65 mcg/day  
   >=16 years   15-80 mcg/day  
Total (N+E):
   Age   Range  
   <1 year   0-13 mcg/day  
   1 year   1-21 mcg/day  
   2-3 years   4-35 mcg/day  
   4-9 years   8-55 mcg/day  
   10-15 years   14-85 mcg/day  
   >=16 years   15-100 mcg/day  
Dopamine:
   Age   Range  
   <1 year   0-85 mcg/day  
   1 year   10-140 mcg/day  
   2-3 years   40-260 mcg/day  
   4-9 years   65-400 mcg/day  
   10-15 years   65-400 mcg/day  
   >=16 years   65-400 mcg/day  
Creatinine:
   Male:   1.0-2.0 g/24 hours  
   Female:   0.8-1.8 g/24 hours  

Performed
Setup: Monday, Wednesday, Friday
Reported: 2-4 days

Turnaround Time4-6 days
Test CodeUCATE
CPT-4 Code (s)
82384, 82570
LOINC Codes
Urine volume: 19153-6
Creatinine 24 hr: 2162-6
total catecholamine: 2058-6
Norepinephrine: 2668-2
Epinephrine: 2232-7
Dopamine: 2218-6

Warde
Medical
Laboratory