COMPREHENSIVE VIRUS DETECTION

Specimen Required
Specimen source required.
Swab specimens in viral transport medium.
Bronchoalveolar lavage/wash in sterile leak-proof container. 3.0 mL (1.5 mL minimum).
CSF and body fluids undiluted in sterile leak-proof container. 3.0 mL (1.5 mL minimum).
Nasal washes in sterile, leak proof sterile container. 3.0 mL (1.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Body fluids undiluted in sterile leak-proof container. 1.0 mL (1.0 mL minimum).
Biopsy/tissue specimens should be ordered for Tissue Comprehensive Virus Detection (TCVD)
Stool in IATA-approved screw-capped container. 2.0 mL (1.0 mL minimum) liquid stool or marble size solid stool.
Undiluted urine in an IATA-approved screw-capped container. 2.0 mL (1.0 mL minimum).
Blood in EDTA (lavender top) tube. 3.0 mL (2.0 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).

Alternate Specimen
Stool in Cary Blair medium.
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Rectal swabs and stool preserved in formalin, SAF, or PVA.
Stool specimens received with diapers, tissue paper, tongue depressors, sticks, and other objects.
Serum will not be tested due to decreased virus concentrations.
Urine in Grey-Top (boric acid) urine vacutainers. Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacterial transport media, Stewart medium (Culturettes) and specimens in bacteriological blood culture media.
Dry swabs, wooden swabs, calcium alginate swabs, and swabs in gel transports.
Specimens received in non-sterile or leaking containers will not be tested.

Methodology
Source-driven panels. Polymerase Chain Reaction

Stability
Non Tissue or Biopsy
Room Temperature (18-25°C): 4 hours;
Refrigerated (2-8°C): 7 days;
Frozen (-20°C): 7 days
Frozen (-70°C): 3 months
Tissue/Biopsy Only
Refrigerated (2-8°C): 3 days
Frozen (-20°C): 1 month
Frozen (-70°C): 3 months

Reference Range
Negative

Performed
Sunday-Saturday

Turnaround Time2 days
Test CodeCVD
CPT-4 Code (s)
Depending on specimen source, testing may include:
87798 Adenovirus PCR
87496 CMV PCR
87498 Enterovirus PCR
87529 x 2 HSV1 and HSV2 PCR
87502 Influenza A & B PCR
87798 Rhinovirus PCR
87634 RSV PCR
87798 VZV PCR
87798 x 2 Norovirus PCR
87631 Parainfluenza PCR
LOINC Codes
Adenovirus PCR   39528-5  
CMV PCR   5000-5  
Enterovirus PCR   29591-5  
HSV1   16130-7  
HSV2   16131-5  
Influenza A PCR   34487-9  
Influenza B PCR   40982-1  
Rhinovirus PCR   40990-4  
RSV PCR   40988-8  
VZV PCR   11483-5  
Norovirus PCR #1   54905-5  
Norovirus PCR #2   54906-3  
Parainfluenza PCR #1   29908-1  
Parainfluenza PCR #2   29909-9  
Parainfluenza PCR #3   29910-7  
Source   31208-2  

Warde
Medical
Laboratory