CYTOMEGALOVIRUS DNA, QUANTITATIVE, PCR

Specimen Required
Draw blood in a lavender EDTA tube. Centrifuge and separate plasma from cells within 24 hours of collection. Send 2.0 mL plasma (1.0 mL minimum) in a screw-capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested. Test performed at Warde Medical Laboratory.

Rejection Criteria
The following specimens will not be tested:
• Whole Blood Specimens (plasma must be separated prior to reciept)
• Specimens collected in any gel-based plasma-separation media
• Shared specimens
• Specimens that do not meet the collection storage/handling conditions criteria above

Methodology
This test uses the polymerase chain reaction to amplifiy conserved regions of the cytomegalovirus (CMV) UL34 and UL80.5 genes. Real-time detection and quanitfication are used to determine the viral concentration. The analytical measurement range is 50-156 million IU/mL (1.70 to 8.9 log 10 IU/mL). The qualitative limit of detection is 31 IU/mL (1.49 log 10 IU/mL) compared to the WHO International Standard.
Specimens reported as POSITIVE but <50 IU/mL contain detectable levels of CMV DNA but the viral load is below the limit of quantitation. A negative result does not rule out infection.

Stability
Room temperature: 24 hours; Refrigerated: 5 days; Frozen: 30 days

Reference Range
Not Detected
<50 IU/mL
<1.70 log10 IU/mL

Performed
Monday-Friday

Turnaround Time3 days
Test CodeCMVQA
CPT-4 Code (s)
87497
LOINC Codes
Cytomegalovirus DNA, Qualitative:   5000-5  
Cytomegalovirus DNA, Quantitative:   34720-3  
Log Cytomegalovirus:   53763-9  
CMV DNA (cp/mL):   33006-8  

Warde
Medical
Laboratory