CYTOMEGALOVIRUS PCR, QUALITATIVE

Test Documentation
Specimen Required
Specimen source required
Requires dedicated sample
Swab specimens (ocular, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in sterile leak-proof container, 1.0 mL (0.5 mL minimum).
Sputum Undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)
CSF and body fluids undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)
Nasal washes in leak proof sterile container, 1.0 mL (0.5 mL minimum)
Urine undiluted in sterile leak-proof container, 2.0 mL (1.0 mL minimum)
Blood in EDTA (lavender top) tube, 2.0 mL (1.0 mL minimum)
EDTA plasma (lavender top), 1. mL (0.5 mL minimum)
Bone Marrow in EDTA, 1.0 mL (0.5 mL minimum)
Amniotic Fluid, 1.0 mL (0.5 mL minimum) in sterile leak-proof container

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine Vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Stool
Serum
Specimens received in non-sterile or leaking containers will not be tested

Methodology
This test utilizes the polymerase chain reaction to amplify a 60 base pair region of the cytomegalovirus (CMV) DNA polymerase gene. Real-time amplification and detection are used to determine if the specimen is positive or negative for CMV. A negative result does not rule out infection. The analytical sensitivity of this assay is 600 copies/mL.

Stability
Room Temperature (18-25°): 3 days
Refrigerated (2-8°C): 2 weeks
Frozen (-20°C): 2 weeks
Frozen (-70°C): 3 months

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeCYPCR
CPT-4 Code (s)
87496
LOINC Codes
CMV   5000-5  
Source   31208-2  

Warde
Medical
Laboratory