EPSTEIN-BARR VIRUS DNA PCR, QUALITATIVE, PLASMA

Specimen Required
Draw blood in a lavender EDTA tube. Centrifuge and separate plasma from cells within 6 hours of collection. Send 2.0 mL plasma (1.0 mL minimum) frozen in a screw-capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.

Rejection Criteria
Serum, heparinized plasma, whole blood, specimens collected using gel-based separation tubes, and specimens submitted to repeated freeze-thaw cycles.

Methodology
This test uses the polymerase chain reaction to amplify regions of the Epstein Barr Virus BLLF1 gene. Real-time detection and quantification are used to determine the viral concentration. The analytical measurement range is 500 to 5 million IU/mL (2.7 to 6.7 log (10) IU/mL). The qualitative limit of detection is 50 IU/mL (1.7 log (10) IU/mL).
Specimens reported as "Detected" but <500 IU/mL, contain detectable levels of EB Virus DNA, but the viral load is below the limit of quantification. A "Not Detected" result does not rule out infection.

Stability
Room temperature: 8 hours; Refrigerated: 3 days; Frozen (-20°C): 30 days; Frozen (-70°C): 6 months

Reference Range
Not detected

Performed
Monday-Friday

Turnaround Time3 days
Test CodeEBQL
CPT-4 Code (s)
87798
LOINC Codes
5005-4

Warde
Medical
Laboratory