EPTEIN-BARR VIRUS DNA PCR, QUANTITATIVE, PLASMA

Specimen Required
Draw blood in a lavender EDTA tube. Centrifuge and separate plasma from cells within 6 hours of collection. Send 2.0 mL plasma (1.0 mL minimum) frozen in a screw-capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.

Rejection Criteria
Serum, heparinized plasma, whole blood, specimens collected using gel-based separation tubes, and specimens submitted to repeated freeze-thaw cycles.

Methodology
Polymerase Chain Reaction (PCR)
This test uses the polymerase chain reaction to amplify regions of the Epstein Barr Virus BLLF1 gene. Real-time detection and quantification are used to determine the viral concentration. The analytical measurement range is 500 to 5 million IU/mL (2.7 to 6.7 log (10) IU/mL). The qualitative limit of detection is 50 IU/mL (1.7 log (10) IU/mL).
Specimens reported as "DETECTED" but <500 IU/mL, contain detectable levels of EB Virus DNA but the viral load is below the limit of quantification. A "NOT DETECTED" result does not rule out infection.

Stability
Room temperature: 8 hours; Refrigerated: 3 days; Frozen (-20°C): 30 days; Frozen (-70°C): 6 months

Reference Range
EBV Qualitative: Not Detected
EBV Quantitative: <500 IU/mL
Log EBV: <2.7 log(10) IU/mL

Performed
Monday-Friday

Turnaround Time3 days
Test CodeEBVQN
CPT-4 Code (s)
87799
LOINC Codes
Epstein-Barr Virus DNA, Qualitative:   5005-4  
Epstein-Barr Virus Dna, Quantitative:   43730-1  
Log Epstein-Barr Virus DNA:   53774-6  

Warde
Medical
Laboratory