FOURTH GENERATION HIV AG/AB (DIAGNOSTIC) REFLEX TO HIV1/HIV2 SUPPLEMENTAL ASSAY

Test Documentation
Components
Samples ordered for HIV1/HIV2 Supplemental confirmation assay (HIVD) must have a prior reactive HIV1/2 3rd or 4th generation screen. The screening assay may be performed at the client laboratory or at Warde Medical Laboratory

Specimen Required
Draw blood in a plain red-top tube. Centrifuge, separate and send 1.0 mL serum (0.5 mL minimum) refrigerated in a screw-capped plastic vial.

Alternate Specimen
Serum: SST
Plasma: EDTA, sodium or lithium heparin, sodium citrate

Rejection Criteria
Gross hemolysis

Methodology
Enzyme Immunoassay

Stability
Room Temperature: 48 hours; Refrigerated: 7 days; Frozen: 14 days

Reference Range
Non-reactive

Performed
HIV1/HIV2 A: Sunday-Friday
HIV1/2D: Monday, Wednesday, Friday

Turnaround TimeHIVA: 3 days for non-reactive screen. Reactive screen will be run in duplicate on the next scheduled run day. If reactive, sample is reflexed to HIVD. If HIV1/HIV2 Supplemental assay is discordant from initial screening results, then HIV nucleic acid testing may be indicated. This requires a dedicated sample, specimens used in other assays cannot be tested.
Test CodeHIVA, HIVD
CPT-4 Code (s)
87389 HIVA
86701, 86702 HIVD at additional cost if performed
LOINC Codes
HIV 1, 2 screen   56888-1  
HIV1   7917-8  
HIV2   7919-4  

Warde
Medical
Laboratory