HEPATITIS C VIRUS GENOTYPE

Specimen Required
Draw blood in lavender EDTA tube. Centrifuge and separate plasma from cells within 6 hours of collection. Send 1.5 mL plasma (1.0 mL minimum) frozen in a screw-capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.

Alternate Specimen
Serum: Red-top

Rejection Criteria
The following specimens will not be tested:
Any gel-based plasma separation media.
Shared specimens.
Specimens subjected to repeated freeze-thaw cycles.
Specimens that do not meet the storage/handling criteria above

Methodology
The Hepatitis C Genotype assay utilizes multiplex polymerase chain reaction (PCR) amplification of two segments of the HCV genome - the 5' non-translated region and the 3' core region. Amplicon detection and genotyping is accomplished using the eSensor HCVg Direct Test from Genmark. This procedure can detect and distiguish HCV genotypes 1a, 1b, 2 a/c, 2b, 3, 4, 5, and 6. This procedure cannot provide a genotype for specimens with viral loads <625 IU/mL.

Stability
Room temperature: Unacceptable; Refrigerated: 3 days; Frozen (-20°C): 30 days

Reference Range
By report

Performed
RNA Isolation: Sunday, Tuesday, Thursday
Reporting: Monday, Wednesday, Friday

Turnaround Time5 days
Test CodeHCVGO
CPT-4 Code (s)
87902
LOINC Codes
32286-7

Warde
Medical
Laboratory