HER-2/NEU GENE AMPLIFICATION (FISH)

Specimen Required
Send formalin fixed paraffin embedded tissue at room temperature. Specimen must be fixed in 10% neutral buffered formalin. Fixation between 6 and 72 hours and cold ischemic time of less than 1 hour is recommended. Pathology report must be included with specimen.
Pathology report should include: Physician identification, specimen identifiers (case and block number), specimen site and type, tissue processing used (routine or microwave), cold ischemic time, type of fixative, time and duration of fixation, pathologic diagnosis, and IHC score if performed elsewhere.

Alternate Specimen
5 micron (4 micron minimum) sections collected on each of 6 charged slides.

Rejection Criteria
Non-invasive tumors

Methodology
Fluorescent in situ Hybridization

Stability
Room Temperature: Indefinite; Refrigerated: Indefinite; Frozen: Unacceptable

Reference Range
By report

Performed
Sunday-Friday

Turnaround Time5-7 days
Test CodeHERNF
CPT-4 Code (s)
88377
LOINC Codes
HER-2/neu Fish   31150-6  
Specimen source   31208-2  

Warde
Medical
Laboratory