HERPES SIMPLEX VIRUS 1 & 2 PCR, QUALITATIVE

Test Documentation
Specimen Required
Specimen source required.
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
CSF and body fluids undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Blood in EDTA (lavender top) tube. 2.0 mL (1.0 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA.
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool samples
Serum
Urine

Methodology
Herpes Simplex Virus (HSV) detection is performed by a real-time PCR amplification and detection procedure. The HSV type 1 assay detects a genetic sequence at the glycoprotein G/J junction while the HSV-2 assay targets a portion of the glycoprotein G gene. The analytical sensitivity of this assay is 50 copies/mL. A negative result does not rule out infection.

Stability
Room Temperature (18-25°C): 3 days; Refrigerated (2-8°C): 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeHSV12
CPT-4 Code (s)
87529 x 2
LOINC Codes
HSV1   16130-7  
HSV2   16131-5  
Source   31208-2  

Warde
Medical
Laboratory