HIV-1 GENOTYPE (RT, PI, INTEGRASE INHIBITORS)

Specimen Required
Draw blood in a lavender EDTA. Centrifuge and separate plasma from cells within 24 hours. Send 5.0 mL plasma (1.6 mL minimum) frozen in a screw-capped plastic vial.

Rejection Criteria
Gross hemolysis, lipemia, serum, whole blood, heparinized plasma

Methodology
Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) - Sequencing

Stability
Room temperature: 24 hours; Refrigerated: 5 days; Frozen: 35 days

Reference Range
By report

Performed
Monday-Saturday

Turnaround Time6-11 days
Test CodeHIV1I
CPT-4 Code (s)
87900, 87901, 87906
LOINC Codes
Date Viral Load Collected   33882-2  
Raltegravir Resistance   72525-9  
HIV-1 Genotype:   48558-1  
Elvitegravir Resistance:   72536-7  
Dolutegravir Resistance:   72857-6  

Warde
Medical
Laboratory