HIV-1 RNA, QUALITATIVE

Specimen Required
Draw blood in one 7.0 mL lavender EDTA tube. Centrifuge and separate from cells within 6 hours of collection. Send 3.0 mL plasma (2.5 mL minimum) frozen in a screw-capped plastic vial. Dedicated specimens are required.

Alternate Specimen
Plasma: ACD A

Rejection Criteria
Whole Blood EDTA, PPT tubes, shared specimens and specimens subjected to repeated freeze-thaw cycles.

Methodology
Abbott RealTime HIV-1 system uses an in vitro-polymerase chain reaction (RT-PCR) assay for the detection and quantitation of a diverse group M subtypes A-H as well as group O and N isolates. The qualitative limit of detection is 20 copies/mL (1.30 log copies/mL).

Stability
After separation from cells: Room Temperature: Uncceptable; Refrigerated (2-8°C): 3 days; Frozen (-20°C): 3 days; Frozen (-70°C): 6 weeks

Reference Range
Negative

Performed
Monday-Friday

Turnaround Time3-5 days
Test CodeHV1QL
CPT-4 Code (s)
87535
LOINC Codes
25835-0

Warde
Medical
Laboratory