HIV-1 RNA, ULTRA QUANTITATIVE

Specimen Required
Draw blood in one 7.0 lavender EDTA tube. Centrifuge and separate from cells within 6 hours of collection. Send 3.0 mL plasma (2.5 mL minimum) frozen in a screw-capped plastic vial. Dedicated specimens are required.

Alternate Specimen
Plasma: ACD

Rejection Criteria
Serum specimens; Shared specimens; Specimens subjected to repeated freeze-thaw cycles; Specimens that do not meet the handling/storage criteria listed above; Gel based plasma separation media (PPT)

Methodology
Abbott RealTime HIV-1 system uses an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection and quantitation of the diverse group M subtypes A-H as well as group O and N isolates.
The lower limit of quantitation is 40 copies/mL (1.60 log copies/mL) and the upper limit of quantitation is 10,000,000 copies/mL (7.0 log copies/mL). The qualitative limit of detection is 20 copies/mL (1.30 log copies/mL). Specimens reported as DETECTED but <40 copies/mL contain detectable levels of HIV-1 RNA even though the viral load is below the limit of quantitation.

Stability
Room Temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 3 days
Frozen (-70°C): 6 weeks

Reference Range
HIV-1 RNA:   Not detected  
HIV-1 RNA Quantitative:   <40 copies/mL  
LOG HIV CP U/mL:   <1.6  

Performed
Monday-Friday

Turnaround Time3-5 days
Test CodeHIVUL
CPT-4 Code (s)
87536
LOINC Codes
25835-0 HIV-1 RNA, Qualitative
20447-9 HIV-1 RNA, Quantitative
29541-0 Log HIVCP U/mL

Warde
Medical
Laboratory