IMMUNE DEFICIENCY PANEL, COMPREHENSIVE

Test Documentation
Components
This lymphocyte subset panel provides a broad overview of the cellular and humoral immune system function. Flow cytometry is not recommended in the evaluation of autoimmune disease, fibromyalgia, or chronic fatigue syndrome.
Includes quantitation of CD3, CD4, CD8 (T-cell), CD19 (B-cell), and CD16/56 (natural killer) markers, and a CD4:CD8 ratio.

Specimen Required
Draw blood in a lavender EDTA tube. Send 4.0 mL whole blood (1.0 mL minimum) room temperature. Ship within 24 hours of collection.

Rejection Criteria
Heparinized samples, ACD samples, refrigerated and frozen samples, and clotted specimens

Methodology
Flow Cytometry

Stability
Room Temperature: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Range
<16 years:   By report  
Adult >16 years:
Pan T Cell (CD3):   55-86%  
Pan T Cell (CD3):   704-2138 C/UL  
T Helper Cell (CD4):   35-66%  
T Helper Cell (CD4):   443-1471 C/UL  
T Suppressor Cell (CD8):   9-37%  
T Suppressor Cell (CD8):   190-832 C/UL  
B Cell (CD19):   4-25%  
B Cell (CD19):   100-524 C/UL  
Natural Killer Cell (CD16+56):   3-24%  
Natural Killer Cell (CD16+56):   60-500 C/UL  
T4/T8 Ratio (CD4:CD8):   1.0-3.7 Ratio  

Performed
Monday-Friday

Turnaround Time3 days
Test CodeTBNK
CPT-4 Code (s)
86360, 86355, 86357, 86359
LOINC Codes
Pan T Cell (CD3)%   20599-7  
Pan T Cell (CD3) C/UL   20598-9  
T Helper Cell (CD4) %   8123-2  
T Helper Cell (CD4) C/UL   24467-3  
T Suppressor Cell (CD8) %   32518-3  
T Suppressor cell (CD8) C/UL   54218-3  
B Cell (CD19) %   20620-1  
B Cell (CD19) C/UL   20592-2  
Natural Killer Cell (CD16+56)%   20620-1  
Natural Killer Cell (CD16 +56)C/UL   26561-1  
T4/T8 Ratio (CD4:CD8)   Not available  

Warde
Medical
Laboratory