INFLUENZA A GENOTYPING

Specimen Required
Specimen source required.
NP and Throat swabs in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar Lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Cell culture medium in an IATA-approved sterile screw-capped container.

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.

Methodology
This procedure is intended for testing influenza A positive specimens. This procedure uses reverse-transcriptase PCR and real-time detection to differentiate seasonal A/H1, A/H3 types, and Novel 2009 H1N1. This test will not differentiate the A/H3N2v strain from the seasonal A/H3 strain. Genotyping is helpful when selecting antiviral therapy because each hemagglutinin type has a different antiviral susceptibility pattern. This procedure should not be used as a general influenza screening tool because these tests will not detect all circulating influenza types.

Stability
Room Temperature (18-25°C): 3 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months

Reference Range
Seasonal H1: Not detected
Seasonal H3: Not detected
Novel 2009 H1N1: Not detected

Performed
Monday-Friday

Turnaround Time2 days
Test CodeFLAGN
CPT-4 Code (s)
87502, 87503
LOINC Codes
H12009   55465-9  
Seasonal H1   49521-8  
Seasonal H3   49524-2  
Source   31208-2  

Warde
Medical
Laboratory