INFLUENZA A GENOTYPING

Test Documentation
Specimen Required
Specimen source required.
NP and Throat swabs in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar Lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Cell culture medium in an IATA-approved sterile screw-capped container.

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.

Methodology
This procedure is intended for testing influenza A positive specimens. This procedure uses reverse-transcriptase PCR and real-time detection to differentiate seasonal A/H1, A/H3 types, and Novel 2009 H1N1. This test will not differentiate the A/H3N2v strain from the seasonal A/H3 strain. Genotyping is helpful when selecting antiviral therapy because each hemagglutinin type has a different antiviral susceptibility pattern. This procedure should not be used as a general influenza screening tool because these tests will not detect all circulating influenza types.

Stability
Room Temperature (18-25°C): 3 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months

Reference Range
Seasonal H1: Not detected
Seasonal H3: Not detected
Novel 2009 H1N1: Not detected

Performed
Monday-Friday

Turnaround Time2 days
Test CodeFLAGN
CPT-4 Code (s)
87502, 87503
LOINC Codes
H12009   55465-9  
Seasonal H1   49521-8  
Seasonal H3   49526-7  
Source   31208-2  

Warde
Medical
Laboratory