INFLUENZA VIRUS A AND B PCR

Test Documentation
Specimen Required
Specimen source is required.
NP and Throat swabs in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum).

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma

Methodology
Influenza A and Influenza B virus detecton is performed by reverse transcription of Influenza A and Influenza B RNA sequences and real-time PCR amplification and detection. The Influenza A assay detects the presence of a conserved region of the matrix gene and the Influenza B assay detects a conserved region of the NS1 protein. Available nucleic acid sequence information suggest that the Influenza A test will detect all known Influenza A subtypes including avian Influenzas. However, this method cannot distinguish avian from non-avian subtypes. The analytical sensitivity of this assay is 100 copies/mL.

Stability
Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months.

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeFLPCR
CPT-4 Code (s)
87502
LOINC Codes
Influenza A   34487-9  
Influenza B   40982-1  
Source   31208-2  

Warde
Medical
Laboratory