JAK2 GENE, (V617F) MUTATION, QUANTITATIVE, PCR

Specimen Required
Draw blood in an EDTA lavender tube. Send 7.0 mL whole blood (3.0 mL minimum) refrigerated.

Alternate Specimen
Whole blood in K2EDTA (pink top) tubes are acceptable

Rejection Criteria
Serum, plasma, heparinized whole blood, tissue specimens

Methodology
This procedure uses real-time polymerase chain reaction amplification and detection to quantify the percentage of white blood cells containing the V617F (G>T at position 617) point mutation in the autoinhibitory pseudokinase domain of the JAK2 protein. The lower limit of quantitation is 0.1% (1 to 1000 cells).

Stability
Room Temperature: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Reference Range
Percent of WBCs with V617F Mutation: <0.1%

Performed
Tuesday, Friday

Turnaround Time4 days
Test CodeJAK2Q
CPT-4 Code (s)
81270
LOINC Codes
JAK2   43399-5  
JAK2%   53761-3  

Warde
Medical
Laboratory