JAK2 V617F CASCADING REFLEX

Specimen Required
Send 5.0 mL whole blood or 3.0 mL bone marrow collected in EDTA lavender top tube. Send room temperature.

Alternate Specimen
Whole blood: sodium heparin tube
Extracted DNA from CLIA-certified laboratory
Bone marrow: 3.0 mL (1.0 mL minimum), EDTA or sodium heparin

Methodology
Polymerase Chain Reaction-based DNA Sequencing

Stability
Whole blood, Bone marrow, Cell pellet: Room temperature: 7 days; Refrigerated: 7 days; Frozen: Unacceptable
EXTRACTED DNA:
Room temperature: 7 days; Refrigerated: 14 days; Frozen: 1 year

Reference Range
See report

Performed
Monday-Sunday

Turnaround Time6-9 days
Test CodeJAKCR
CPT-4 Code (s)
81270 (add if reflexed 81219 CALR; 81279 Exon 12 JAK2; 81338 MPL; 81479 CSF3R at additional fees)
JAK2 V617F ZBX5Q
CALR EXON 9 ZBX5M
JAK2 EXON 12 ZB11R
MPL EXON 10 ZB11T
CSF3R EXON 14/17 ZB11S
LOINC Codes
Clinical Indication   55752-0  
Specimen Source   31208-2  
Block/Specimen ID   52463-7  
JAK2 V617F Mutation   43399-5  
CALR Exon 9 Mutation   77174-1  
JAK2 Exon 12 Mutation   55300-8  
MPL Exon 10 Mutation   62947-7  
CSF3R Exon 14/17 Mutation   92674-1  
Gene   48018-6  
Amino Acid   48005-3  
Mutation Frequency   81258-6  
Mutation Type   48019-4  
Exon   47999-8  
Nucleotide Change   48004-6  
Reference   81256-0  
Interpretation   50398-7  
Assay Details   8266-9  

Warde
Medical
Laboratory