JAK2 V617F CASCADING REFLEX

Specimen Required
Send 5.0 mL whole blood or 1.0 mL bone marrow collected in EDTA lavender top tube. Send room temperature.

Alternate Specimen
Whole blood or bone marrow aspirate: sodium heparin tube
Extracted DNA from CLIA-certified laboratory

Methodology
Polymerase Chain Reaction-based DNA Sequencing

Stability
Whole blood, Bone marrow, Cell pellet: Room temperature: 7 days; Refrigerated: 7 days; Frozen: Unacceptable
EXTRACTED DNA:
Room temperature: 7 days; Refrigerated: 14 days; Frozen: 1 year

Reference Range
See report

Performed
Monday-Sunday

Turnaround Time6-9 days
Test CodeJAKCR
CPT-4 Code (s)
81270, plus 81219 CALR; 81403 Exon 12 JAK2; 81402 MPL; 81479 CSF3R as appropriate, at additional fees
JAK2 V617F ZBX5Q
CALR EXON 9 ZBX5M
JAK2 EXON 12 ZB11R
MPL EXON 10 ZB11T
CSF3R EXON 14/17 ZB11S
LOINC Codes
Clinical Indication   55752-0  
Specimen Source   31208-2  
Block/Specimen ID   52463-7  
JAK2 V617F Mutation   43399-5  
CALR Exon 9 Mutation   77174-1  
JAK2 Exon 12 Mutation   55300-8  
MPL Exon 10 Mutation   62947-7  
CSF3R Exon 14/17 Mutation   92674-1  
Gene   48018-6  
Amino Acid   48005-3  
Mutation Frequency   81258-6  
Mutation Type   48019-4  
Exon   47999-8  
Nucleotide Change   48004-6  
Reference   81256-0  
Interpretation   50398-7  
Assay Details   8266-9  

Warde
Medical
Laboratory