LEAD AND ZINC PROTOPORPHYRIN, INDUSTRIAL EXPOSURE

Specimen Required
Draw blood in a dark blue EDTA tube. Send 7.0 mL whole blood (2.0 mL minimum) refrigerated. PROTECT FROM LIGHT.
A completed lead requisition must accompany sample.

Alternate Specimen
Whole blood: Lavender EDTA, K2EDTA

Rejection Criteria
Lead: Clotted samples
ZPP: Hemolyzed & clotted samples

Methodology
Atomic Absorption Spectrophotometry/Fluorometry

Stability
ZPP: Room Temperature: 8 days; Refrigerated: 8 weeks; Frozen: Unacceptable
Lead: Room temperature: 7 days; Refrigerated: 1 month; Frozen: Not recommended

Reference Range
COMPONENTS   REFERENCE INTERVAL  
Lead, Whole Blood   0-16 years: <10 µg/dL  
   16-150 years: 0-24.9 µg/dL  
Zinc Protoporphyrin (ZPP), Whole Blood 0-69 µmol/mol heme
Zinc Protoporphyrin (ZPP), Whole Blood 0-40 µg/dL
Interpretive Data:
Action required for workers with elevated Lead Values OSHA, Occupational Exposure to Lead, 1978
No. of Tests   (µg/dL)   Action Required  
1   greater than or equal to 40.0   Notification of workers in writing; medical examination of worker and consultation.  
3   greater than or equal to 50.0   Removal of worker from job with potential lead exposure.  
1   greater than or equal to 60.0   Removal of worker from job with potential lead exposure.  
2   greater than or equal to 40.0   Reinstaement of worker in job with potential lead exposure is based upon symptoms and medical evaluation.  
OSHA requirements in effect since 1978 call for the measurement of whole blood lead and zinc protoporphyrins (ZPP) (NCCLS document C42-A, Nov. 1996) to evaluate the occupational exposure to lead. OSHA requires ZPP whole blood testing to be reported in units of µg/dL. Federal lead construction standards require a worker to be notified and removed from the job at levels of 50 µg/dL and higher, until a physician authorizes return.

Performed
Lead: Sunday-Friday
ZPP: Sunday, Tuesday, Thursday

Turnaround Time2-4 days
Test CodeZPPI
CPT-4 Code (s)
83655 (Lead), 84202 (Zinc Protoporphyrin)
LOINC Codes
Lead, whole blood   5671-3  
ZPP, umol/MOL HEME   29763-0  
ZPP, Whole blood   33007-6  

Warde
Medical
Laboratory