MGMT PROMOTER METHYLATION DETECTION
Specimen Required
Rejection Criteria
Methodology
Stability
Reference Range
Performed
| Tumor Tissue: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block and 5 unstained 5 micron slides. |
Rejection Criteria
| Specimens fixed in alternative fixatives | |||
| Heavy metal fixatives (B-4 or B-5) | |||
| Decalcified specimens | |||
| Less than 25% tumor |
Methodology
| Polymerase Chain Reaction/MassARRAY/MAL-DI-TOF |
Stability
| Room temperature: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable |
Reference Range
| See report |
Performed
| DNA Isolation: Sunday - Saturday; Assay: Varies |
| 10 - 14 days | |||||
| MGPMD | |||||
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