MPL MUTATION ANALYSIS

Specimen Required
Draw blood in a lavender EDTA. Send 5.0 mL whole blood (1.0 mL minimum) refrigerated.

Alternate Specimen
Bone marrow: Lavender EDTA, 1.0 mL (0.5 mL minimum)
Cell pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimens source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.

Rejection Criteria
Gross hemolysis

Methodology
Polymerase Chain Reaction-based DNA Sequencing

Stability
Room temperature: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Reference Range
By report

Performed
Tuesday-Saturday

Turnaround Time6-8 days
Test CodeMPLQL
CPT-4 Code (s)
81402
LOINC Codes
Clinical Indication:   55752-0  
Specimen Source:   31208-2  
Block/Specimen ID:   52463-7  
MPL Exon 10 Mutation:   62948-5  
Gene:   19146-0  
Amino Acid:   19146-0  
Mutation Frequency:   19146-0  
Mutation Type:   19146-0  
Exon:   19146-0  
Nucleotide Change:   19146-0  
Reference:   75608-0  
Interpretation:   48767-8  
Assay Details:   49549-9  

Warde
Medical
Laboratory