NOROVIRUS GROUP 1 & 2 DETECTION BY RT-PCR

Specimen Required
Source required.
Send 1.0 mL stool (0.5 mL minimum) refrigerated in a sterile IATA-approved screw-capped plastic container.

Alternate Specimen
Stool in Cary Blair preservative. Swab specimens (rectal) in viral transport medium. Swabs in culturettes must be transferred to viral transport within 24 hours of collection.

Rejection Criteria
Stool or rectal swabs preserved in formalin, SAF, or PVA.
Specimens received in non-sterile or leaking containers.
Stool specimens received on diapers, tisssue paper, tongue depressors, sticks or other objects. Emesis will not be tested.
Culturettes older than 24 hours.

Methodology
Reverse-Transcription Polymerase Chain Reaction
Norovirus virus detection was performed by reverse transcriptase polymerase chain reaction (PCR) amplification of a portion of the viral RNA polymerase gene and real-time detection using TaqMan detection probes. This method will detect genogroup 1 and genogroup 2 noroviruses. The analytical sensitivity of this assay is approximately 100 virus copies/assay. Positive results should be correlated with clinical presentation as Noroviruses are excreted in the stool for 2-3 weeks after clinical symptoms resolve. A negative result does not rule out infection.

Stability
Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeNOPCR
CPT-4 Code (s)
87798 x 2
LOINC Codes
Norovirus #1   54905-5  
Norovirus #2   54906-3  
Specimen source   31208-2  

Warde
Medical
Laboratory