Specimen Required
NP and throat swabs in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in sterile leak-proof container 1.0 mL (0.5 mL minimum)
Sputum undiluted in sterile leak-proof container 1.0 mL (0.5 mL minimum)
Nasal aspirates in vacuum trap 1.0 mL (0.5 mL minimum)
Nasal washes in leak proof sterile container 1.0 mL (0.5 mL minimum)

Alternate Specimen
The Laboratory Director or Supervisor must approve testing specimens other than those listed above.

Rejection Criteria
The following specimens will not be tested:
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Whole blood, serum, plasma

This test utilizes a reverse transcriptase real-time PCR procedure to detect type-specific areas within the HN gene of human parainfluenza. The analytical sensitivity of this assay is 200 copies/mL. A "Not detected" result does not rule out infection.
This test uses commercial reagents that have not been approved or cleared by the FDA. The FDA has determined that such clearance or approval is not necessary. The performance characteristics of this procedure were determined by Warde Medical Laboratory. This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.

Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Parainfluenza 1: Not detected
Parainfluenza 2: Not detected
Parainfluenza 3: Not detected


Turnaround Time2 days
Test CodePIPCR
CPT-4 Code (s)
87631 (or 87798 each virus performed, if fewer than three performed)
Parainfluenza #1   29908-1  
Parainfluenza #2   29909-9  
Parainfluenza #3   29910-7  
Source   31208-2