PERTUSSIS PCR PANEL

Specimen Required
Specimen source required. Collect nasopharyngeal swab (Dacron or Rayon swabs only), place in viral transport media, and send refrigerated.

Alternate Specimen
Nasal aspirate, 1.0 mL (0.5 mL minimum) in vacuum trap.
Nasal wash, 1.0 mL (0.5 mL minimum) in an IATA-approved screw-capped sterile plastic container.

Rejection Criteria
Specimens in other transport media
Specimens in Regan Lowe media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, wooden swabs and swabs in gel transports
Specimens in culturettes
Throat and anterior nasal swabs (due to unacceptably low rates of detection)
Specimen received in non-sterile or leaking containers.

Methodology
This Pertussis Panel utilizes the multi-target detection approach recommended by the Centers for Disease Control and Prevention (MMWR 2007; 56(33):837). This panel includes real-time PCR testing for the IS481 gene of B. pertussis, the p1S1001 gene from B. parapertussis, and the h1S1001 gene from B. holmseii. Reflex testing for the pertussis toxin gene is used as needed to detect dual infections with B. parapertussis and B. holmseii. The analytical sensitivity of this panel is approximately 3 colony forming units/assay.

Stability
Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Not detected

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeBPPCR
CPT-4 Code (s)
87798 x 3
LOINC Codes
Specimen Source   31208-2  
B. pertussis   43913-3  
B. parapertussis   42588-4  
B. holmseii   62427-0  

Warde
Medical
Laboratory