PORPHYRINS, FRACTIONATION AND QUANTITATION, URINE

Specimen Required
Collect 24 hour urine. Refrigerate and protect from light during collection. Mix well and send 4.0 mL urine (2.0 mL minimum) frozen in an amber screw-capped plastic vial. PROTECT FROM LIGHT. Record 24 hour urine volume on test requisition and specimen label.

Alternate Specimen
Random urine

Methodology
Quantitative High Performance Liquid Chromatography

Stability
Room Temperature: Unacceptable; Refrigerated: 4 days; Frozen: 1 month

Reference Range
Uroporphyrin: 0-4 µmol/mol CRT
Heptacarboxylate Porphyrin: 0-2 µmol/mol CRT
Coproporphyrin I: 0-6 µmol/mol crt
      Coproporphyrin III: 0-14 µmol/mol crt  
Creatinine:
By report

Performed
Monday-Friday

Turnaround Time5-7 days
Test CodeUPORA
CPT-4 Code (s)
84120
LOINC Codes
Creatinine, Urine - per 24h   2162-6   mg/d  
Hours Collected   30211-7   hr  
Porphyrin Urine Interpretation   49292-6  
Creatinine, Urine - per volume   20624-3   mg/dL  
Heptacarboxylate - ratio to CRT   38175-6   umol/mol CRT  
Uroporphyrin - ratio to CRT   32043-2   umol/mol CRT  
Coproporphyrin 1 - ratio to CRT   45313-4   umol/mol CRT  
Coproporphyrin III- ratio to CRT   45315-9   umol/mol CRT  
Total Volume   19153-6   mL  

Warde
Medical
Laboratory