PYRUVIC ACID

Specimen Required
Patient Prep: Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm and hand before or during collection. Draw the specimen without the use of a tourniquet or within 3 minutes of applying the tourniquet, but before releasing the tourniquet. Draw blood in a green (sodium or lithium heparin). Immediately after blood sample has been obtained, add 1.0 mL whole blood to a chilled colleciton tube containing 2 mL 8% (W/V) perchloric acid. Mix well for 30 seconds and place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1500 g. Decant 2.0 mL supernatant (1.0 mL minimum) and send frozen in screw-capped plastic vial. Tubes available from laboratory.

Rejection Criteria
Specimens not prepared as indicated

Methodology
Quantitative Enzymatic

Stability
Room Temperature: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks

Reference Range
0.03-0.107 mmol/L

Performed
Sunday-Saturday

Turnaround Time3-5 days
Test CodePYRUA
CPT-4 Code (s)
84210
LOINC Codes
14121-8

Warde
Medical
Laboratory