RBC MEMBRANE EVALUATION, B

Specimen Required
Testing requires a whole blood EDTA specimen from patient, a whole blood EDTA control specimen and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol). Draw Monday - Thursday only. Specimens must be received at Warde Medical Lab the day of collection.
Patient:
Draw blood in lavender top EDTA tube. Send 4.0 mL blood refrigerated in original tube. In addition, collect 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol).
Normal shipping control:
Draw blood in lavender EDTA tube from a healthy, non-smoking person at the same time as patient. Send 4.0 mL blood clearly labeled as control specimen.

Rejection Criteria
Gross hemolysis, clotted

Methodology
Varies by test

Stability
Room temperature: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Range
> or = 12 months:
0.50 g/dL NaCl (unincubated): 3-53% hemolysis
0.60 g/dL NaCl (incubated): 14 - 74% hemolysis
0.65 g/dL NaCl (incubated): 4 - 40% hemolysis
0.75 g/dL NaCl (incubated): 1 -11 % hemolysis
Interpretive report provided
Reference values not established for patiens <12 months of age.

Performed
Monday - Saturday

Turnaround Time 5 - 7 days
Test CodeRBCME
CPT-4 Code (s)
85557, 88184, 85060
LOINC Codes
Osmotic Fragility, RBC   34964-7  
Osmotic Fragility, 0.50 g/dL NaCl   23915-2  
Osmotic Fragility, 0.60 g/mdL NaCl   23918-6  
Osmotic Fragility, 0.65 g/dL NaCl   23920-2  
Osmotic Fragility, 0.75 g/dL NaCl   23921-0  
Osmotic Fragility Comment   59466-3  
Shipping Control Vial   40431-9  
Spherocytosis Interpretation   50595-8  
Reviewed By   18771-6  
Band 3 Fluoresence Staining, RBC   Not available  
Peripheral Blood Smear Review   59465-5  

Warde
Medical
Laboratory