RESPIRATORY SYNCYTIAL VIRUS (RSV) PCR

Test Documentation
Specimen Required
Specimen source is required.
Throat plus NP swab in viral transport medium
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal Aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal Washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).

Alternate Specimen
The laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma

Methodology
Reverse transcription of conserved regions of the respiratory syncytial virus (RSV) RNA genome, polymerase chain reaction amplification, and real-time detection. This assay detects the presence of a conserved region of the RNA polymerase gene from RSV A and RSV B. This method cannot distinguish the A and B subtypes. The analytical sensitivity of this assay is 100 copies/mL. A negative result does not rule out infection.

Stability
Room Temperature (18-25°C): 3 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months

Reference Range
Negative

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeRVPCR
CPT-4 Code (s)
87798
LOINC Codes
Specimen source   31208-2  
RSV PCR   40988-8  

Warde
Medical
Laboratory