RHINOVIRUS PCR

Specimen Required
Specimen source required.
NP and throat swabs in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL)
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum)
Nasal washes in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum)

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or Probetec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Whole blood
Serum
Plasma

Methodology
This test utilizes a reverse transcriptase real-time PCR procedure to detect the 5'-nontranslated region of the rhinovirus genome. This procedure will detect most, but not all of the >200 rhinovirus types. This procedure cannot differentiate the individual rhinovirus types. This procedure is significantly more sensitive than culture for rhinovirus detection. The analytical sensitivity of this assay is 200 copies/mL. A negative result does not rule out infection.

Stability
Room Temperature: 3 days; Refrigerated: 14 days; Frozen (-20°C): 14 days; Frozen (-70°C): 3 months

Reference Range
Negative

Performed
Monday-Saturday

Turnaround Time2 days
Test CodeRNPCR
CPT-4 Code (s)
87798
LOINC Codes
Specimen source   31208-2  
Rhinovirus PCR   40990-4  

Warde
Medical
Laboratory