VANILLYLMANDELIC ACID (VMA) - URINE, 24 HOUR

Specimen Required
Collect 24 hour urine (see collection instructions below). Send 25 mL acidified urine (10.0 mL minimum) refrigerated in a screw-capped plastic urine cup. Record total volume on test requisition and specimen label. 24 hour urine collection: The bladder must be emptied at the time the collection is to begin, and this urine is discarded. Thereafter all urine must be collected until the end of the scheduled time. Preferred method of collection for 24 hour collection: Collect urine with 25 mL of 6N HCl at start of collection to maintian pH between 1 and 3. Refrigerate urine during collection (critical). Mix the collection well before removing aliquot. Send 25.0 mL urine (10.0 mL minimum) refrigerated in a screw-capped plastic urine container. Record total volume on container label.

Alternate Specimen
Alternate method of 24 hour collection with preservative: Refrigerate urine during collection (critical) and add 25.0 mL 6N HCl to the entire collection within 4 hours of collection completion. Mix collection well, and check pH to ensure pH is between 1 and 3. Send a 25.0 mL urine aliquot (10.0 mL minimum) refrigerated in a screw-capped plastic urine container. Record total volume on container label. Alternate method of 24 hours collection without preservative: Collect 24 hours urine and keep refrigerated throughout collection. Mix well and send 25.0 mL urine (10.0 mL minimum) refrigerated in a screw-capped plastic urine container. Record total volume on container label.

Rejection Criteria
Samples with pH <1

Methodology
High Performance Liquid Chromatography

Stability
With preservative: Room Temperature: 10 days; Refrigerated: 14 days; Frozen: 1 year
Without preservative: Room Temperature: Unacceptable; Refrigerated: 7 days; Frozen: 14 days

Reference Range
3-5 years: 1-2.6 mg/day
6-9 years: 2.0-3.2 mg/day
10-15 years: 2.3-5.2 mg/day
16-83: 1.8-6.7 mg/day
Creatinine:
Males:   1.0-2.0 g/24 hours  
Females:   0.8-1.8 g/24 hours  

Performed
Setup: Monday, Wednesday, Friday
Reported: 2-4 days

Turnaround Time4-6 days
Test CodeUVMA
CPT-4 Code (s)
84585, 82570
LOINC Codes
Total volume   19153-6  
Creatinine   2162-6  
VMA urine   3122-9  

Warde
Medical
Laboratory