VANILLYLMANDELIC ACID (VMA) - URINE, 24 HOUR

Test Documentation
Specimen Required
Collect 24 hour urine (see collection instructions below). Send 25 mL acidified urine (10.0 mL minimum) refrigerated in a screw-capped plastic urine cup. Record total volume on test requisition and specimen label.
24 hour urine collection: The bladder must be emptied at the time the collection is to begin, and this urine is discarded. Thereafter all urine must be collected until the end of the scheduled time.
Preferred method of collection for 24 hour collection: Collect urine with 25 mL of 6N HCl at start of collection to maintain pH between 1 and 3. Refrigerate urine during collection (critical). Mix the collection well before removing aliquot. Record total volume on test request and on container label. Send refrigerated 25 mL aliquot (10 mL minimum).

Alternate Specimen
Alternate method of collection for 24 hour collection: Refrigerate urine during collection (critical) and add 25 mL 6N HCl to the entire collection within 4 hour of completing. Mix the collection well before checking the pH. Ensure pH is between 1-3. Record total volume on test request and on container label. Send refrigerated 25 mL aliquot (10 mL minimum).

Methodology
High Performance Liquid Chromatography

Stability
With preservative: Room Temperature: 3 days; Refrigerated: 7 days; Frozen: 1 month
Without preservative: Room Temperature: 24 hours; Refrigerated: 24 hours; Frozen: 7 days
Preservative must be added before sample is submitted for testing.

Reference Range
3-5 years: 1-2.6 mg/day
6-9 years: 2.0-3.2 mg/day
10-15 years: 2.3-5.2 mg/day
16-83: 1.8-6.7 mg/day
Creatinine:
Males:   1.0-2.0 g/24 hours  
Females:   0.8-1.8 g/24 hours  

Performed
Setup: Monday, Wednesday, Friday
Reported: 2-4 days

Turnaround Time4-6 days
Test CodeUVMA
CPT-4 Code (s)
84585, 82570
LOINC Codes
Total volume   19153-6  
Creatinine   2162-6  
VMA urine   3122-9  

Warde
Medical
Laboratory