VIRUS CULTURE

Test Documentation
Components
This procedure wil not reliably detect cytomegalovirus (CMV), enteroviruses, noroviruses, respiratory syncytial virus (RSV), and varicella zoster (VZV), Comprehensive Virus Detection (CVD) or the individual PCR test is recommended. See CYPCR, EVCR, NOPCR, RSPCR and VVPCR.
CVD is strongly recommended for blood, any body fluid, bronchoalveolar lavage/wash, and aspirates. Urine specimens are acceptable for Mumps detection only. CSF is not acceptable for Virus Culture.

Specimen Required
Specimen source required.
Swab specimens in viral transport medium.
Biopsy/tissue specimens in saline or viral transport medium. (snap frozen -20°C).
Nasal washes in sterile, leak proof sterile container, 2.0 mL (1.0 mL minimum).
Nasal aspirates in vacuum trap, 1.0 mL (0.5 mL minimum).
Body fluids undiluted in sterile leak-proof container. 2.0 mL (1.0 mL minimum).
Stool in sterile leak-proof container, 2.0 mL (1.0 mL minimum). Liquid stool or marble size solid stool. Stool in Cary blair medium.
Undiluted urine in sterile leak-proof container, 2.0 mL (1.0 mL minimum), for Mumps only.
Bronchoalveolar lavage/wash in sterile leak-proof container, 2.0 mL (1.0 mL minimum).
Note: CVD is the preferred method.
Blood in EDTA (lavender top) tube, 3.0 mL (2.0 mL minimum). Note: CVD is the preferred method.

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
CSF - Please reorder as Comprehensive Virus Detection (CVD).
Urine specimens for culture other than Mumps or Measles virus - Please reorder as CVD.
Urine (for Mumps) in Grey-Top (boric acid) urine vacutainers
Rectal swabs or stool preserved in formalin, SAF, or PVA.
Stool specimens received with diapers, tissue paper, tongue depressors, sticks, and other objects containing stool.
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacterial transport media, Stewart medium (Culturette) and specimens in bacteriological blood culture media.
Dry swabs, wooden swabs, calcium alginate swabs, and swabs in gel transports.
Tissues received in transport media that has not been frozen (-20°C).
Any specimen other than tissues frozen at temperatures warmer than -70°C.
Specimens received in non-sterile or leaking containers.

Methodology
Standard tube cultures, hemagglutination, hemadsorption, acid lability testing, virus neutralization, polymerase chain reaction and/or monoclonal antibody staining. Viruses that can be isolated include most Adenoviruses, most Enteroviruses, Herpes simplex virus, Influenza A and B, Measles virus, Mumps Virus, Parainfluenza (1, 2 and 3), Respiratory syncytial virus and Rhinovirus.

Stability
Room Temperature (18-25°C): 4 hours
Refrigerated (2-8°C): 7 days (except tissues)
Frozen (-20°C): Do Not Freeze at -20°C (except tissues);
Frozen (-70°C): Do Not Freeze at -70°C (except tissues)
Tissue and Biopsy Only
Room Temperature (18-25°C): 4 hours
Refrigerated (2-8°C): Not Acceptable
Frozen (-20°C): 1 month
Frozen (-70°C): 3 months

Reference Range
No growth

Performed
Sunday-Saturday

Turnaround TimeUp to 14 days
Test CodeVC
CPT-4 Code (s)
87252 Culture inoculation
87253 additional testing, identification, at an additional charge
LOINC Codes
6584-7

Warde
Medical
Laboratory