Vedolizumab QN with Antibodies, Serum

Specimen Required
Patient Preparation: 12 hours prior to testing: Do not take multivitamins or dietary supplements containing biotin (Vitamin B7).
Nivolumab must be discontinued at least 4 weeks prior to testing.
Draw blood in red top. Centrifuge within 2 hours of collection and send 1.5 mL serum (0.75 mL minimum) refrigerated in a screw-capped plastic vial.

Alternate Specimen
Serum: SST

Rejection Criteria
Gross hemolysis, lipemia or icterus samples

Methodology
Vedolizumab Quant: Liquid Chromatography-Mass Spectrometry (LC-MS/MS)
Vedolizumab Antibodies: Electrochemiluminescent Bridging Immunoassay

Stability
Room temperature: Unacceptable; Refrigerated: 28 days; Frozen: 28 days

Reference Range
VEDOLIZUMAB QUANTITATION: Vedolizumab lower limit of quantitation = 2.0 mcg/mL
VEDOLIZUMAB ANTIBODIES: Antibodies to vedolizumab: <9.8 ng/mL

Performed
Vedolizumab Quant: Monday, Thursday; Vedolizumab Antibodies: Tuesday, Friday

Turnaround Time8-16 days
Test CodeVEDOZ
CPT-4 Code (s)
80280 (Vedolizumab), 82397 (Antibodies)
LOINC Codes
Vedolizumab QN, S   90805-3  
Vedolizumab Ab, S   86899-2  
VEMAB Interpretation   59462-2  

Warde
Medical
Laboratory