B27 – “HLA-B27 Screening” replaced by B27M – “HLA-B27 Identification by PCR”

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Inactivated/Replace Test

Effective Date

01/13/2026

Included in Report

WML Test Directory Update | January 2026

Replacement Test

HLA-B27 IDENTIFICATION by PCR

Inactivated Test Details

Mnemonic

B27

Ordering Code

3080980

CPT-4 Codes(s)

86812

Replacement Test Details

Mnemonic

B27M

Ordering Code

3000462

CPT-4 Codes(s)

81374

Turnaround Time
3 - 5 days
Performed
Monday, Thursday
Legacy Code
B27M
Example Report
Download

Specimen Information

Collect

Lavender EDTA

Specimen Preparation

Draw blood in a lavender EDTA. Send 4.0 mL whole blood.

Alternate Specimen

Whole blood: ACD A

Minimum Volume

0.5 mL

Transport Temperature

Refrigerated

Rejection Criteria

Serum, plasma, heparinized whole blood, tissue

Stability

Room temperature: 3 days
Refrigerated: 7 days
Frozen: 30 days

Methodology

Polymerase Chain Reaction (PCR)
more

Reference Range

See report
more

Performing Laboratory

Warde Medical Laboratory
more

New York DOH Approval Status

No
more

Interface Map for B27M

Test Name Ordering Code Result Code Component Name LOINC Code AOE Prompt Units
HLA-B27 Identification by PCR3000462
3000463HLA-B27 AlleleNo

The CPT codes provided are for general informational purposes only and are based on an interpretation of AMA guidance. It is the sole responsibility of the billing party to determine CPT codes. Any questions regarding coding should be directed to the paying party.

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