Test Details

Mnemonic

AGAS

Ordering Code

3805600

CPT-4 Code(s)

82657

Specimen Information

Collect

Red top

Specimen Preparation

Centrifuge, separate serum from cells and send 2.0 mL serum in a screw capped plastic vial. Sex of patient is required for interpretation.

Alternate Specimen

Serum separator tube (SST)

Minimum Volume

0.2 mL

Transport Temperature

Frozen

Rejection Criteria

Gross hemolysis, gross lipemia, gross icterus

Stability

Room temperature: Unacceptable
Refrigerated: 24 hours
Frozen: 14 days

Methodology

Alpha-galactosidase is a lysosomal enzyme active at an acidic pH. The enzyme hydrolyzes artificial substrates such as 4-methylumbelliferyl and alpha-D galactopyranoside. The 4-methylumbelliferone liberated is measured by fluorometry.
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Reference Range

0.074 - 0.457 U/L
Note: Results from this assay are not useful
for carrier determination. Carriers
usually have levels in the normal range.
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Performing Laboratory

Mayo Clinic Laboratories
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New York DOH Approval Status

Yes
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