Test Details

Mnemonic

CVD

Ordering Code

3099000

CPT-4 Code(s)

Depending on specimen source, testing may include: 87798 Adenovirus PCR 87496 CMV PCR 87498 Enterovirus PCR 87529 x 2 HSV1 and HSV2 PCR 87502 Influenza A & B PCR 87798 x 2 Norovirus 1 & 2 87631 Parainfluenza 1-3 PCR 87798 Rhinovirus PCR 87634 RSV PCR 87798 VZV PCR

Specimen Information

Collect

Variable specimen types

Specimen Preparation

Swab specimens in viral transport medium.
Bronchoalveolar lavage/wash in sterile, leak-proof container - 3.0 mL (1.5 mL minimum).
CSF and body fluids undiluted in sterile, leak-proof container - 3.0 mL (1.5 mL minimum).
Nasal washes in sterile, leak-proof sterile, container - 3.0 mL (1.5 mL minimum).
Nasal aspirates in vacuum trap - 1.0 mL (0.5 mL minimum).
Body fluids undiluted in sterile, leak-proof container - 1.0 mL (1.0 mL minimum).
Biopsy/tissue specimens should be ordered for Tissue Comprehensive Virus Detection (TCVD).
Stool in IATA-approved screw-capped container - 2.0 mL (1.0 mL minimum) liquid stool or marble size solid stool.
Undiluted urine in an IATA-approved screw-capped container - 2.0 mL (1.0 mL minimum).
Blood in EDTA (lavender top) tube - 3.0 mL (2.0 mL minimum).
Bone Marrow in EDTA - 1.0 mL (0.5 mL minimum).

Alternate Specimen

Stool in Cary Blair medium.
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.

Minimum Volume

Determined by specimen type

Transport Temperature

Varies by specimen type, see stability

Rejection Criteria

Rectal swabs and stool preserved in formalin, SAF, or PVA.
Stool specimens received with diapers, tissue paper, tongue depressors, sticks, and other objects.
Serum will not be tested due to decreased virus concentrations.
Urine in Grey-Top (boric acid) urine vacutainers. Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacterial transport media, Stewart medium (Culturettes) and specimens in bacteriological blood culture media.
Dry swabs, wooden swabs, calcium alginate swabs, and swabs in gel transports.
Specimens received in non-sterile or leaking containers will not be tested.

Stability

Room temperature (18-25°C): 4 hours;
Refrigerated (2-8°C): 7 days;
Frozen (-20°C): 7 days
Frozen (-70°C): 3 months

Methodology

Source-driven panels. Polymerase Chain Reaction
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Reference Range

Negative
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Performing Laboratory

Warde Medical Laboratory
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