Test Details

Mnemonic

UBKQN

Ordering Code

3092700

CPT-4 Code(s)

87799

Specimen Information

Collect

Random urine
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Specimen Preparation

Send 2.0 mL urine undiluted and frozen in a sterile IATA-approved screw-capped plastic container. Dedicated specimens are required. Specimens used in other assays will not be tested.
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Minimum Volume

1.0 mL
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Transport Temperature

Frozen
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Rejection Criteria

Shared specimens, specimens submitted to repeated freeze-thaw cycles, urine in Grey-top (boric acid) urine Vacutainers, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above
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Stability

Room temperature: 8 hours
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
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Methodology

This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection & quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL with an extended range up to 50 billion copies/mL with dilution (2.10 to 10.70 log(10 copies/mL). The qualitative limit to detection is 100 copies/mL (2.00 log(10) copies/mL). Specimens reported as "DETECTED" byt <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
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Reference Range

BKV QUAL: Not detected (NOT DET)
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
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Performing Laboratory

Warde Medical Laboratory
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