Test Details

Mnemonic

HSV12

Ordering Code

3091740

CPT-4 Code(s)

87529 x 2

Specimen Information

Collect

Variable specimen types
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Specimen Preparation

Specimen source required.
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
CSF and body fluids undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Blood in EDTA (lavender top) tube. 2.0 mL (1.0 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).
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Alternate Specimen

The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
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Minimum Volume

Determined by specimen type
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Transport Temperature

Varies with specimen type, see stability
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Rejection Criteria

Rectal swabs preserved in formalin, SAF, or PVA.
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool samples
Serum
Urine
E-swabs
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Stability

Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Do not freeze whole blood or bone marrow.
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Methodology

Herpes Simplex Virus (HSV) detection is performed by a real-time PCR amplification and detection procedure. The HSV type 1 assay detects a genetic sequence at the glycoprotein G/J junction while the HSV-2 assay targets a portion of the glycoprotein G gene. The analytical sensitivity of this assay is 50 copies/mL. A negative result does not rule out infection.
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Reference Range

Not detected
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Performing Laboratory

Warde Medical Laboratory
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