Test Details

Mnemonic

FLPCR

Ordering Code

3091830

CPT-4 Code(s)

87502

Specimen Information

Collect

Variable specimen types
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Specimen Preparation

Specimen source is required.
NP and Throat swabs in viral transport medium.
If sending one sample for FLPCR and COVW (COVID testing) please send a minimum of 1.5 mL.

Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. Send 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw-capped plastic container. Send 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum).

NP/OP swab in Phosphate Buffered Saline or Sterile Saline.
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Alternate Specimen

The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
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Minimum Volume

Determined by specimen type
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Transport Temperature

Varies by specimen type, see stability
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Rejection Criteria

Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma
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Stability

Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
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Methodology

Influenza A and Influenza B virus detection is performed by reverse transcription of Influenza A and Influenza B RNA sequences and real-time PCR amplification and detection. The Influenza A assay detects the presence of a conserved region of the matrix gene and the Influenza B assay detects a conserved region of the NS1 protein. Available nucleic acid sequence information suggest that the Influenza A test will detect all known Influenza A subtypes including avian Influenzas. However, this method cannot distinguish avian from non-avian subtypes. The analytical sensitivity of this assay is 100 copies/mL.
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Reference Range

Not detected
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Performing Laboratory

Warde Medical Laboratory
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