Test Details

Mnemonic

NOPCR

Ordering Code

3092440

CPT-4 Code(s)

87798 x 2

Specimen Information

Collect

Random stool
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Specimen Preparation

Source required. Send 1.0 mL stool refrigerated in a sterile IATA-approved screw-capped plastic container.
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Alternate Specimen

Stool in Cary Blair preservative.
Swab specimens (rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
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Minimum Volume

0.5 mL
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Transport Temperature

Refrigerated
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Rejection Criteria

Stool or rectal swabs preserved in formalin, SAF, or PVA.
Specimens received in non-sterile or leaking containers.
Stool specimens received on diapers, tisssue paper, tongue depressors, sticks or other objects. Emesis will not be tested.
Culturettes older than 24 hours.
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Stability

Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
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Methodology

Reverse-Transcription Polymerase Chain Reaction
Norovirus virus detection was performed by reverse transcriptase polymerase chain reaction (PCR) amplification of a portion of the viral RNA polymerase gene and real-time detection using TaqMan detection probes. This method will detect genogroup 1 and genogroup 2 noroviruses. The analytical sensitivity of this assay is approximately 100 virus copies/assay. Positive results should be correlated with clinical presentation as Noroviruses are excreted in the stool for 2-3 weeks after clinical symptoms resolve. A negative result does not rule out infection.
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Reference Range

Not detected
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Performing Laboratory

Warde Medical Laboratory
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