Test Details
Mnemonic
ALDR
Ordering Code
1003990
CPT-4 Code(s)
82088, 84244
Specimen Information
Patient Preparation
Angiotensin Converting Enzyme (ACE) Inhibitors should be avoided, if possible, for at least 48 hours prior to collection. Spironlactone (Aldactone) should be discontinued 4 - 6 weeks prior to testing. Patient should be on a normal sodium diet.
Collect
Aldosterone: Serum separator tube (SST) and
Direct Renin: Lavender EDTA
Direct Renin: Lavender EDTA
Specimen Preparation
Aldosterone:
Centrifuge, separate serum from cells and send 1.0 mL in a screw capped plastic vial. Attach the serum label to this sample.
Direct Renin: Do not prechill collection tubes. Centrifuge, separate plasma from cells immediately and send 1.5 mL plasma frozen in a screw capped plastic vial. CRITICAL FROZEN.
Centrifuge, separate serum from cells and send 1.0 mL in a screw capped plastic vial. Attach the serum label to this sample.
Direct Renin: Do not prechill collection tubes. Centrifuge, separate plasma from cells immediately and send 1.5 mL plasma frozen in a screw capped plastic vial. CRITICAL FROZEN.
Alternate Specimen
Aldosterone:
Serum: Red top
Direct Renin: No alternate specimen
Serum: Red top
Direct Renin: No alternate specimen
Minimum Volume
Aldosterone: 0.5 mL
Direct Renin: 1.0 mL
Direct Renin: 1.0 mL
Transport Temperature
Serum (Aldosterone): Refrigerated
Plasma (Direct Renin): CRITICAL FROZEN
Plasma (Direct Renin): CRITICAL FROZEN
Rejection Criteria
Aldosterone sample: plasma, gross hemolysis, gross lipemia, incorrect sample identification.
Direct Renin: Hemolysis, gross lipemia, nonfrozen plasma, serum, plasma from dark blue EDTA collection tubes, incorrect sample type identification.
Direct Renin: Hemolysis, gross lipemia, nonfrozen plasma, serum, plasma from dark blue EDTA collection tubes, incorrect sample type identification.
Stability
Aldosterone:
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: 30 days
Renin:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: 30 days
Renin:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Methodology
Aldosterone: Chemiluminescence
Direct Renin: Chemiluminescence
Ratio: Calculation
Direct Renin: Chemiluminescence
Ratio: Calculation
Reference Range
Aldosterone: Upright: ≤39.2 ng/dL
Supine: ≤23.2 ng/dL
Aldosterone/Direct Renin Ratio: 0.1 - 3.7
Renin: 3.1 - 57.1 pg/mL
Supine: ≤23.2 ng/dL
Aldosterone/Direct Renin Ratio: 0.1 - 3.7
Renin: 3.1 - 57.1 pg/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No