Test Details
Mnemonic
HBVQN
Ordering Code
3041500
CPT-4 Code(s)
87517
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Centrifuge and separate plasma from cells within 6 hours of collection. Send 3.0 mL plasma in a screw capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.
Minimum Volume
2.5 mL
Transport Temperature
Refrigerated
Rejection Criteria
Serum, all gel-based plasma separation tubes, shared specimens, specimens subjected to repeated freeze-thaw cycles, specimens that do not meet the storage/handling conditions criteria above
Stability
Room temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Methodology
This procedure utilizes a real-time Polymerase Chain Reaction (PCR) procedure from Abbott Molecular. The amplification target is a conserved region in the terminal third of the hepatitis B surface antigen gene. The lower limit of quantitation is 10 IU/mL (1.00 Log IU/mL) and the upper limit of quantitation is 1 billion IU/mL (9.00 Log IU/mL).
The qualitative limit of detection is 10 IU/mL (1.00 Log IU/mL). Specimens reported as "DETECTED" but <10 IU/mL contain detectable levels of hepatitis B DNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
The qualitative limit of detection is 10 IU/mL (1.00 Log IU/mL). Specimens reported as "DETECTED" but <10 IU/mL contain detectable levels of hepatitis B DNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
Reference Range
Hepatitis B DNA: Not detected
Hepatitis B DNA, Quantitative: <10 IU/mL
LOG HBV IU/mL: <1.00
Hepatitis B DNA, Quantitative: <10 IU/mL
LOG HBV IU/mL: <1.00
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes