May 2022 WML Test Directory Update

Due to reagent shortages, we are changing the performing laboratory and updating test requirements.

CPT Code(s): 86316

Collect: Serum separator tube (SST)

Specimen Preparation: Allow specimen to clot completely at room temperature. Separate serum from cells within 2 hours of collection. Send 1.0 mL serum in a screw capped plastic vial. 

Minimum Volume: 0.5 mL 

Transport Temperature: Refrigerated

Rejection Criteria: Plasma. Hemolyzed specimens

Alternate Specimen: Red top

Methodology: Quantitative Immunoassay 

Performed: Monday, Wednesday, Friday

Stability:

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 1 year (avoid repeated freeze/thaws cycles)

Reference Range: ≤12.7 ng/mL

Performing Laboratory: ARUP Reference Laboratory

Update to rejection criteria.

Rejection Criteria: Serum separator tube (SST), Plasma separator tube (PST), glass container 

Update to name.

Name: Encephalopathy, Autoimm/Paraneo, S

Update to name.

Name: Epilepsy, Autoimm/Paraneo, CSF 

Update to name.

Name: Epilepsy, Autoimm/Paraneo, S

Update to reference range.

Reference Range: See report

Update to test name, specimen requirements, and performed days.

Name: Circulating Immune Complex, C1q Binding

Specimen Preparation: Allow sample to clot completely (up to 1 hour). Centrifuge, separate serum from cells within 30 minutes and send 1.0 mL in a screw capped plastic vial. CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Performed Days: Monday, Thursday, Saturday

Update to methodology, rejection criteria, and reference range.

Methodology: Complement Fixation (CF)/Immunodiffusion/Enzyme-Linked Immunosorbent Assay (ELISA)

Rejection Criteria: Contaminated, hemolyzed, icteric or lipemic specimens, body fluids other than serum.

Reference Range: See report

Update to specimen requirements and methodology.

Specimen Preparation: Collect Cerebrospinal fluid (CSF) and send 2.0 mL fluid in a screw capped plastic vial. Mark specimen plainly as “acute” or “convalescent”. 

Methodology: Complement fixation/Immunodiffusion

Update to rejection criteria, methodology, and reference range.

Rejection Criteria: Other body fluids, contaminated, hemolyzed, icteric, or lipemic specimens.

Methodology: Complement fixation/Immunodiffusion

Reference Range:

Coccidioides Antibody by ID             Not detected
Coccidioides Antibody by CF             Less than 1:2

Physician attestation form requirement updates.

Specimen Information: This germline genetic test requires a physician attestation form that patient consent has been received if the ordering medical facility is located in AK, DE, FL, GA, IA, MA, MN, NV, NJ, OR, SD or VT or test is performed in MA.

Update to CPT codes.

CPT Code(s): 80326, 80347, 80364, 80355 (G0481)

Update to rejection criteria and reference range.

Rejection Criteria: Other body fluids, contaminated, hemolyzed, icteric or lipemic specimens

Reference Range: See report 

Update to specimen requirements, alternate specimen, stability, and reference range.

Specimen Preparation: Send 3.0 whole blood.

Minimum Volume: 1.0 mL

Alternate Specimen: Whole blood: yellow (ACD A or B)

Stability:

Room temperature: 7 days
Refrigerated: 1 month
Frozen: Unacceptable

Reference Range: See report

Update to specimen requirements and rejection criteria.

Specimen Preparation: Centrifuge, separate serum from cells within 2 hours of collection and send 1.0 mL serum in a screw capped plastic vial. Mark specimens as “acute” or “convalescent”.

Rejection Criteria: Plasma, hemolyzed, lipemic, icteric, heat inactivated or turbid specimens, bacterially contaminated. 

Update to stability.

Stability:

Whole blood, Bone marrow:
Room temperature: 24 hours
Refrigerated: 4 days
Frozen: Unaccepatable

Extracted DNA:
Room temperature: 30 days
Refrigerated: Indefinitely
Frozen: Indefinitely