Updated Test

Effective Date

03/29/2022

Updated Test

OXALATE, PLASMA

Specimen Information

Patient Preparation

Previous: Updated To:
Deleted: Added: Patient should be fasting for at least 12 hours prior to collection. Patient should avoid taking vitamin C supplements for 24 hours prior to collection.

Collect

Previous: Updated To:
Deleted: Added: Green sodium heparin

Specimen Preparation

Previous: Updated To:
Deleted: Added: After drawing specimen, immediately place green sodium heparin tube on wet ice. Centrifuge for 10 minutes 3500 rpm within 1 hour of collection. Use of a 4 degree °C refrigerated centrifuge is optimal but not required. Aliquot 5.0 mL plasma in screw capped plastic vial. Adjust the pH of the plasma specimen to a pH of 2.3-2.7 with approximately 10 mcL concentrated (12M) hydrochloric acid (or 20 mcL of 6M HCl) per 1 mL plasma. CRITICAL FROZEN.

Alternate Specimen

Previous: Updated To:
Deleted: Added: Nonacidified specimens can be accepted if the heparinized plasma is properly frozen. A disclaimer will be added in nonacidified plasma, "Sample was received nonacidified and frozen. In nonacidified samples oxalate values may increase spontaneously." Although there can be a small absolute increase of plasma oxalate in nonacidified specimens, this will not change the clinical interpretation.

Minimum Volume

Previous: Updated To:
Deleted: Added: 2.0 mL

Transport Temperature

Previous: Updated To:
Deleted: Added: Critical Frozen

Rejection Criteria

Previous: Updated To:
Deleted: Added: Sample collected in anything other than a green sodium heparin.

Stability

Previous: Updated To:
Added: Room Temperature: Unacceptable
Added: Refrigerated: Unacceptable
Deleted: Added: Frozen: 30 days

Methodology

Previous: Updated To:
Deleted: Added: Enzymatic

Reference Range

Previous: Updated To:
Deleted: Added: ≤ 2.0 mcmol/L
Added: Reference values have not been established for patients younger than 18 years of age or older
Added: than 87 years of age.

Performing Laboratory

Previous: Updated To:
Deleted: Added: MAYO

Days Performed

Previous: Updated To:
Deleted: Added: Monday - Friday

Turn-around Time

Previous: Updated To:
Deleted: Added: 5 - 9 days