FLOW CYTOMETRY, ACUTE PANEL, CSF

While minimum & ideal amounts of sample, transport conditions and times are suggested below, the flow lab will almost always attempt to perform the ordered flow analysis on minimal samples or markedly aged samples. However, in such instances, it may be impossible to render an interpretation due to loss of viability, loss of particular cell types and/or insufficient numbers of diagnostic cells that are analyzable by flow cytometry. Regardless of source please try to deliver sample within 24 hours of collection to Warde Laboratory even if the need for analysis is uncertain. The latter cases can be sent as "Flow Holds" (FHOLD is Soft code). Due to seasonal changes in temperature it is imperative that the proper temperature is maintained during shipping because it directly affects cell viability. For example, specimens requiring refrigeration should be shipped with a refrigerated "cold pack" or room temperature should be insulated in a way to prevent freezing during transit.
Please note: temperature stability may differ by specimen type.

Cerebrospinal fluid (CSF)

Send 1.0 mL Cerebrospinal fluid (CSF) in a screw capped plastic vial.

0.5 mL

Refrigerated

Frozen samples, cracked or leaking containers, sample ≥72 hours from collection

Room temperature: Unacceptable
Refrigerated: 24 hours
Frozen: Unacceptable